Oftentimes one runs sets of 1D NOESY spectra, selectively exciting several peaks in separate experiments. 2D NOESY can be run without significant user intervention, but 1D NOESY requires manual selection of one peak for excitation. It can even be used to determine forward/backward rates for systems in equilibrium on the 0.1 – 5 second timescale (NOESY is called EXSY when used for this purpose). It’s good for answering qualitative structural questions like “is this H axial or equatorial?” and “which of these geminal vinyl Hs corresponds to which NMR multiplet?” It can also be used for structure determination, including quantitative 1H- 1H distance measurement (if done carefully). Please recall that the 1H- 1H NOESY shows which hydrogen atoms are close together in space (under 4 or 5 Å), regardless of the chemical bonds between them. Where to find QR codes at the spectrometer computer Why NOESY?
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